Inhibitory effect of D-penicillamine on degradation of hexosamine-containing substances in the involution of carrageenin granuloma induced by calcium chelate ehtylenediaminetetraacetate.

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Abstract
The effect of Ca-EDTA and D-penicillamine on the content of hexosamine-containing substances in the carrageenin granuloma in rats was tested by means of their daily local injection into preformed granuloma pouch during 3 or 4 days. The administration of Ca-EDTA revealed involution of preformed granuloma and significantly decreased the content of glycosaminoglycans (especially, hyaluronic acid and chondroitin sulfate A) and glycoproteins. When Ca-EDTA and D-penicillamine were simultaneously administered or D-penicillamine was given 8 h after the injection of Ca-EDTA, involution of granulomatous tissue and degradation of both hexosamine-containing substances were completely inhibited. Free N-acetyl-.beta.-glucosaminidase activity in granuloma also increased by treatment with Ca-EDTA, while free enzyme activity recovered to the control level by simultaneous treatment with Ca-EDTA and D-penicillamine. The results were discussed in relation to the mode of action of D-penicillamine as an antirheumatic drug.