Development of Myeloid (Chloro-) Leukemia in Thymectomized C3H Mice Following Inoculation of Lymphatic Leukemia Virus.

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Abstract
Of 60, C3H mice inoculated with passage A leukemic virus at age 3 days and thymectomized a month later, 26 (43%) developed leukemia at 10 months. Among leukemic mice in this group, 8 developed myelogenous leukemia. Of 85 litter-mate control injected simultaneously with the same filtrates but not thymectomized, 93% developed lymphatic leukemia at 3.6 months. Myelogenous leukemia developing in some of the virus-injected and subsequently thymectomized mice could be readily transplanted by cell-graft to adult mice of the same strain mostly reverting to lymphatic form, but in few instances retaining myelogenous character. Preliminary results suggest that myelogenous leukemia could be transmitted by filtrates inoculated into newborn mice, but then lymphatic leukemia was induced. Susceptibility of thymectomized mice to leukemogenic action of passage A virus could be restored by subcutaneous implantation of thymuses from young normal C3H mice. Of 20, C3H mice injected with the leukemic virus, then thymectomized, and which subsequently received subcutaneous implants of normal C3H thymuses, 18 developed leukemia (2 of these were myelogenous), as compared with 35 of 36 non-thymectomized controls that received the same filtrates. Finally, 39, C3H mice were thymectomized at 4 to 6 weeks of age, and inoculated a few days later with passage A leukemic filtrates. None developed leukemia although they were observed until they were 15 months old. Of 41 non-thymectomized adult control mice injected simultaneously with the same filtrates, 63% developed leukemia at 8.7 months average age.