Synthetic protease inhibitors and post-ejaculatory degradation of human semen proteins
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 44 (5) , 433-438
- https://doi.org/10.3109/00365518409083834
Abstract
Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophoretic protein pattern. This invalidates the electrophoretic evaluation of the content in the secretion from the accessory sex glands. Both agarose and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the proteolytic changes and how they could be modified by various synthetic protease inhibitors. Liquefaction of coagulated semen could be inhibited by adding o-phenanthroline directly after ejaculation, whereas neither neutrally buffered Na2EDTA, di-isopropylfluorophosphate (DFP), benzamidine, nor thiol reagents proved effective. Addition of the serine protease inhibitors DFP and benzamidine, o-phenanthroline, and iodoacetamide substantially retarded the proteolytic alterations of the proteins as demonstrated by both agarose electrophoresis and SDS-PAGE. We recommend that electrophoretic protein analysis of human semen be performed on ejaculates collected in vessels containing protease inhibitors. For routine analysis, the addition of benzamidine ensures sufficiently stable proteins to permit reliable electrophoretic analysis of samples stored at room temperature for 4 h.Keywords
This publication has 11 references indexed in Scilit:
- Beta2-Microglobulin, Carcinoembryonic Antigen and Prostatic Acid Phosphatase in Split Ejaculates and Urine Voided Before and After Massage of the ProstateArchives of Andrology, 1983
- Proteins of human semen. I. Two-dimensional mapping of human seminal fluid.Clinical Chemistry, 1981
- Male Reproductive Function and SemenPublished by Springer Nature ,1981
- Biochemical Aspects of the Coagulation and Liquefaction of Human SemenJournal of Andrology, 1980
- Mechanism of liquefaction of the human ejaculate I. Changes of the ejaculate proteinsReproduction, 1979
- Agarose gel electrophoresis.Clinical Chemistry, 1979
- Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.The Journal of cell biology, 1975
- Isolation of a Factor from Normal Human Semen that accelerates Dissolution of abnormally Liquefying SemenFertility and Sterility, 1975
- Modified skatole method for microdetermination of fructose and inulinBiochimica et Biophysica Acta, 1957
- The Distribution of Spermatozoa and of Certain Chemical Constituents in the Human EjaculateJournal of Urology, 1942