Genetic engineering of potato for broad-spectrum protection against virus infection
- 1 November 1996
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 14 (11) , 1597-1601
- https://doi.org/10.1038/nbt1196-1597
Abstract
Transgenic potato plants expressing mutant alleles of PLRV ORF4, the gene for the movement protein pr17 of this luteovirus, were generated for broad-range protection against virus infection. When tested for protection against infection by PLRV, all transgenic lines showed a significant reduction of virus antigen. Potato lines accumulating N- or C-terminally extended PLRV pr17 mutant proteins were resistant to infection by the unrelated potato viruses PVY and PVX. Transgenic lines that did not express protein despite high transcript levels failed to exhibit virus resistance.Keywords
This publication has 19 references indexed in Scilit:
- Aphid Transmission and Systemic Plant Infection Determinants of Barley Yellow Dwarf Luteovirus-PAV are Contained in the Coat Protein Readthrough Domain and 17-kDa Protein, RespectivelyVirology, 1996
- Non-canonical translation mechanisms in plants: efficient in vitro and in planta initiation at AUU codons of the tobacco mosaic virus enhancer sequenceNucleic Acids Research, 1996
- Infections with Various Types of Organisms Stimulate Transcription from a Short Promoter Fragment of the Potatogst1GeneMolecular Plant-Microbe Interactions®, 1996
- Transposon tagging of the maize Glossy2 locus with the transposable element En/SpmThe Plant Journal, 1995
- Pathogen-Derived Resistance to Plant VirusesAnnual Review of Phytopathology, 1995
- A defective movement protein of TMV in transgenic plants confers resistance to multipleviruses whereas the functional analog increases susceptibilityVirology, 1995
- A dysfunctional movement protein of tobacco mosaic virus that partially modifies the plasmodesmata and limits virus spread in transgenic plantsThe Plant Journal, 1993
- Mutational Analysis of the Nucleic Acid-Binding 17 kDa Phosphoprotein of Potato Leafroll Luteovirus Identifies an Amphipathic α-Helix as the Domain for Protein/Protein InteractionsVirology, 1993
- Sense and Antisense RNA-Mediated Resistance to Potato Leafroll Virus in Russet Burbank Potato PlantsMolecular Plant-Microbe Interactions®, 1991
- Plant Pathogenesis-Related Proteins Induced by Virus InfectionAnnual Review of Phytopathology, 1990