Incidence and spread ofHaemophilus influenzaeon an Antarctic base determined using the polymerase chain reaction
- 1 February 1995
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 114 (1) , 93-103
- https://doi.org/10.1017/s0950268800051943
Abstract
SUMMARY: A PCR-based method of detectingHaemophilus influenzaein cultures inoculated from throat swabs was evaluated using samples from groups of laboratory staff and medical students and then applied to samples originating from the closed human community of an Antarctic research station. Suitable PCR primers to anH. influenzaegene (ompP2) were used to amplify the gene from DNA preparations made from mixed growth on chocolate agar with added vancomycin. PCR product was reamplified and subjected to restriction endonuclease digestion to allow temporal and spatial mapping of strains over an 8-month period. Eleven different strains ofH. influenzaewere detected. One particular strain was detected in a third of the base members.Keywords
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