Comparison of Six ‘Third‐Generation’ Tests for the Detection of HBsAg

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Abstract
Six third-generation techniques for the detection of HBsAG [hepatitis B surface antigen] were compared, i.e., the Ausria II-125 and Travenol-RIA radioimmunoassays, the Auszyme and Hepanostika enzyme-linked immunosorbent assays and the Auscell (reversed passive hemagglutination) and a modified Auscell technique applying centrifugation instead of sedimentation. The Ausria II-125 was the most sensitive test, followed by Travenol-RIA, both Auscell techniques, Auszyme and Hepanostika. In the Ausria II-125, Hepanostika and Auscell techniques, 1-2% false-positive results were found in serum and 2% in plasma specimens from blood donors. The Travenol-RIA and Ausyme tests gave an unacceptably high percentage (6-8%) of false-positive results in serum and plasma specimens.