Isolation of a Simian Virus 40-Simian Agent 12 Recombinant Papovavirus That Synthesizes a Hybrid Large Tumour Antigen
- 1 March 1988
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 69 (3) , 683-687
- https://doi.org/10.1099/0022-1317-69-3-683
Abstract
The simian virus 40 (SV40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (T) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. The mutation renders the virus defective. We report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 DNA and the DNA of a related papovavirus, simian agent 12 (SA12). Hyb4001 arose by two homologous recombination events involving crossovers in regions of 7 and 12 base pairs of perfect homology between the two viruses. Hyb4001 synthesizes a hybrid T antigen with the amino-terminal 382 residues encoded by SV40, residues, 383 to 449 encoded by SA12, and the carboxy-terminal 259 residues encoded by SV40.This publication has 11 references indexed in Scilit:
- Simian agent 12 is a BK virus-like papovavirus which replicates in monkey cellsJournal of Virology, 1985
- Mutations near the carboxyl terminus of the simian virus 40 large tumor antigen alter viral host rangeJournal of Virology, 1985
- Two separable functional domains of simian virus 40 large T antigen: carboxyl-terminal region of simian virus 40 large T antigen is required for efficient capsid protein synthesisJournal of Virology, 1985
- Nonviable mutants of simian virus 40 with deletions near the 3' end of gene A define a function for large T antigen required after onset of viral DNA replicationJournal of Virology, 1983
- Biochemical activities of T-antigen proteins encoded by simian virus 40 A gene deletion mutants.Molecular and Cellular Biology, 1983
- Mutational analysis of simian virus 40 T antigen: isolation and characterization of mutants with deletions in the T-antigen gene.Molecular and Cellular Biology, 1983
- Mutational analysis of simian virus 40 T antigen: stimulation of cellular DNA synthesis and activation of rRNA genes by mutants with deletions in the T-antigen gene.Molecular and Cellular Biology, 1983
- New Properties of Simian Virus 40 Large T AntigenMolecular and Cellular Biology, 1982
- Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.The EMBO Journal, 1982
- Characterization of SA12 as a simian virus 40-related papovavirus of chacma baboonsInfection and Immunity, 1977