Kinetics and regulation of cell-free alkaline phosphatase synthesis
- 1 September 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 143 (3) , 1265-1274
- https://doi.org/10.1128/jb.143.3.1265-1274.1980
Abstract
Regulation of alkaline phosphatase (EC 3.1.3.1) synthesis in a cell-free system from Escherichia coli was observed. Synthesis from transducing phage [.vphi. 80] DNA templates carrying phoA+ occurred in S30 fractions from wild-type or alkaline phosphatase-constitutive mutants. It did not occur in S30 fractions from alkaline phosphatase-negative mutants (phoB). The hybrid gene phoA-lacZ was also subject to phoB control, implying that phoA transcription is regulated. The yield of active alkaline phosphatase per phoA+ gene copy from cell-free synthesis was similar to that of .beta.-galactosidase. Alkaline phosphatase activity took longer to appear than .beta.-galactosidase activity. Synthesis of alkaline phosphatase subunits was not delayed, suggesting that a minimum number of subunits are synthesized before formation of active alkaline phosphatase occurs.This publication has 55 references indexed in Scilit:
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