Primary isolation media for Legionnaires disease bacterium

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Abstract
Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% IsoVitaleX and 1% hemoglobin (MH-IH). This medium was subsequently analyzed to determine the components required to support growth of the LDB. L-Cysteine hydrochloride can replace the IsoVitaleX reagent, and soluble ferric pyrophosphate can replace hemoglobin. A new medium, F-G agar, was formulated incorporating these chemicals. Different culture conditions (oxygen tension, temperature and pH) were also evaluated. The LDB grew optimally at 35.degree. C under 2.5% CO2 on the F-G agar adjusted to pH 6.9. When infected tissues were inoculated onto F-G agar and MH-IH, the F-G agar produced colonies of the LDB more rapidly and in greater numbers than did MH-IH.

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