Isolation and carbohydrate composition of glycopeptides of human apo low-density lipoprotein from normal and type II hyperlipoproteinemic subjects

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Abstract
Glycopeptides were prepared from the delipidized protein of low-density lipoprotein (LDL, d = 1.019–1.063) of three normal and three familial heterozygous type II hyperlipoproteinemic (HLP) subjects. The glycopeptides of all subjects were resolved into three groups by gel filtration on Bio-Gel P6 following papain (EC 3.4.22.2) digestion and initial purification on Bio-Gel P2. In normal individuals the component of largest molecular weight (F-1) contained mannose (Man), N-acetyl glucosamine (GlcNAc), galactose (Gal), and N-acetyl neuraminic acid (NANA) in the respective amounts of 45.9 ± 6.7, 37.3 ± 5.9, 28.6 ± 3.4, and 27.0 ± 3.9 nmol/mg original apoprotein. The group of smallest molecular weight (F-3) contained essentially only Man (25.8 ± 1.5 nmol/mg protein) and GlcNac (3.0 ± 0.4 nmol/mg protein) with traces of Gal and NANA. A group of intermediate molecular weight (F-2) exhibited considerable heterogeneity and contained Man, GlcNAc, Gal, and NANA in the amounts of45.9 ± 5.1, 18.3 ± 1.7, 11.0 ± 1.7, and 7.7 ± 1.2 nmol/mg protein. While the major portion of NANA (78%), Gal (71%), and GlcNAc (64%) was present in F-1, approximately 22% of the total Man was in F-3. No major differences were detected in the carbohydrate composition of the three glycopeptide fractions of LDL apoprotein from normal and Type II subjects.

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