Distribution of Brush-border Membrane Peptidases along the Intestine of Rabbits and Rats: Implication for Site-specific Delivery of Peptide Drugs
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Drug Targeting
- Vol. 1 (3) , 231-236
- https://doi.org/10.3109/10611869308996080
Abstract
Distribution patterns of brush-border membrane dipeptidylpeptidase IV (DPP IV), endopeptidase-24.11, and angiotensin converting enzyme (ACE) activities along the intestine of the rat and rabbit were examined. ACE and endopeptidase-24.11 had a similar distribution profile in the intestine of the rat and rabbit, jejunum > duodenum ∼ jejunoileal junction > ileum > caecum (rat) or ileocaecal junction (rabbit). DPP IV had a more uniform distribution pattern than ACE and endopeptidase-24.11. Its longitudinal distribution patterns in the intestine of the rat and rabbit were slightly different. In the rat intestine, DPP IV activity had the following rank order: ileum ∼ jejunum > jejunoileal junction > duodenum > caecum. In the rabbit, DPP IV had similar activities from the jejunum to the ileocaecal junction whereas its duodenal activity was much lower. The results suggest that the distribution profiles of DPP IV, ACE, and endopeptidase-24.11 are similar in both species.Keywords
This publication has 15 references indexed in Scilit:
- A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of d-glucose and choline transport systemsPublished by Elsevier ,2002
- A survey of membrane peptidases in two human colonic cell lines, Caco-2 and HT-29Biochemical Journal, 1992
- Utilization of Peptide Carrier System To Improve Intestinal Absorption: Targeting Prolidase as a Prodrug-Converting EnzymeJournal of Pharmaceutical Sciences, 1992
- Structural Specificity of Mucosal-Cell Transport and Metabolism of Peptide Drugs: Implication for Oral Peptide Drug DeliveryPharmaceutical Research, 1992
- Isolation of endopeptidase-24.11 (EC 3.4.24.11, “enkephalinase”) from the pig stomachGastroenterology, 1988
- Absorption of an aqueous solution of a new synthetic somatostatin analogue administered to man by gavageEuropean Journal of Clinical Pharmacology, 1987
- Effects of Potential Damaging Agents on the Microclimate-pH in the Rat JejunumJournal of Pharmaceutical Sciences, 1986
- Absorption of 1-deamino-8-D-arginine vasopressin from different regions of the gastrointestinal tract in rabbitsActa Endocrinologica, 1986
- The association between acidification and electrogenic events in the rat proximal jejunumThe Journal of Physiology, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976