Determination of Nitrite by Pre-Column Derivatization and High-Performance Liquid Chromatography
- 1 November 1992
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 25 (11) , 2083-2093
- https://doi.org/10.1080/00032719208020077
Abstract
Nitrite has been determined by reversed-phase high-performance liquid chromatography by its pre-column reaction with 2-aminobiphenyl to give a diazonium salt and with iodide to form 2-iodobiphenyl. Chromatographic analysis was done using a C18-column, a mobile phase of aceto-nitrile - 0.05M sulfuric acid, 60:40 (v/v), detection at 254 nm and using biphenyl as an internal standard. The pre-column derivatization required about 8 min and the chromatographic separation was complete in about 10 min. As low as 8 μg 1−1 of nitrite could be determined with a limit of detection of 2 μg 1−1 Application of the proposed method to river water and pharmaceutical tablets has been demonstrated.Keywords
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