EFFECT OF HIGH-DOSE METHOTREXATE WITH CITROVORUM FACTOR ON HUMAN GRANULOPOIESIS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (10) , 4195-4199
Abstract
The present studies were performed to delineate the effect of high-dose methotrexate with citrovorum factor rescue on the measurable compartments of human granulopoiesis, including peripheral blood cells and bone marrow stem cells committed to granulopoiesis-monocytopoiesis. Parameters of granulopoiesis were determined during the 1st course of high-dose methotrexate with citrovorum factor rescue in 5 patients [being treated for osteogenic sarcoma and a synovioma], and during 4 consecutive courses in 1 patient. The remarkably mild toxicity of high-dose methotrexate with citrovorum factor rescue reported in clinical studies was substantiated on the level of the committed stem cell compartment. The data suggest a higher sensitivity of in vitro colony-forming units in agar culture as compared to the more mature granulopoietic cells and document the exquisite sensitivity and regenerating capacity of erythroid precursors after this type of chemotherapy.