Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
- 1 August 2006
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 61 (6) , 945-957
- https://doi.org/10.1007/s11103-006-0060-5
Abstract
Non-phosphorylating glyceraldehyde- 3-phosphate dehydrogenase (NP-GAPDH) is a conserved cytosolic protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. To investigate the role of this enzyme in plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the NP-GAPDH gene locus. The homozygous mutant was determined to be null respect to NP-GAPDH, as it exhibited undetectable levels of both transcription of NP-GAPDH mRNA, protein expression and enzyme activity. Transcriptome analysis demonstrated that the insertion mutant plant shows altered expression of several enzymes involved in carbohydrate metabolism. Significantly, cytosolic phosphorylating (NAD-dependent) glyceraldehyde-3-phosphate dehydrogenase mRNA levels are induced in the mutant, which correlates with an increase in enzyme activity. mRNA levels and enzymatic activity of glucose-6-phosphate dehydrogenase were also elevated, correlating with an increase in NADPH concentration. Moreover, increased ROS levels were measured in the mutant plants. Down-regulation of several glycolytic and photosynthetic genes suggests that NP-GAPDH is important for the efficiency of both metabolic processes. The results presented demonstrate that NP-GAPDH has a relevant role in plant growth and development.Keywords
This publication has 47 references indexed in Scilit:
- Sugarcane yellow leaf virus infection leads to alterations in photosynthetic efficiency and carbohydrate accumulation in sugarcane leavesFitopatologia Brasileira, 2005
- Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes inEscherichia coliFEMS Microbiology Letters, 2004
- Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coliFEMS Microbiology Letters, 2004
- Differential Regulation of Glucose-6-Phosphate Dehydrogenase Isoenzyme Activities in PotatoPlant Physiology, 2003
- Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant rootsPlant, Cell & Environment, 2003
- Phosphate Starvation Triggers Distinct Alterations of Genome Expression in Arabidopsis Roots and LeavesPlant Physiology, 2003
- A novel C‐terminal proteolytic processing of cytosolic pyruvate kinase, its phosphorylation and degradation by the proteasome in developing soybean seedsThe Plant Journal, 2003
- Significance analysis of microarrays applied to the ionizing radiation responseProceedings of the National Academy of Sciences, 2001
- Unraveling the Role of Mitochondria During Oxidative Stress in PlantsIUBMB Life, 2001
- The role of mitochondrial electron transport during photosynthetic induction. A study with barley (Hordeum vulgare) protoplasts incubated with rotenone and oligomycinPhysiologia Plantarum, 1998