Western Blotting of Native and Denatured Bovine β-Lactoglobulin to Detect Addition of Bovine Milk in Cheese

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Abstract
Western blotting of bovine β-LG is a valid method to detect adulteration by pasteurized bovine milk, by UHT bovine milk, or by heat-denatured bovine whey proteins in cheeses made of milk from other species. Use of PAGE of whey or isoelectric focusing of β-LG isolated from the casein fraction was followed by immunodetection with anti-bovine β-LG antiserum. The selectivity of the antisera to react with native and denatured β-LG was studied. Detection limits of native and denatured β-LG standard solutions were 10 and 50 pg/μl, respectively. Immunoblotting of the native-PAGE plates of whey proteins from cheese allows detection of bovine heat-denatured whey proteins or pasteurized bovine milk added to cheese even at 1% can be detected by either Western blotting methods.Peer reviewe