THE ONTOGENY OF INSULIN SECRETION IN THE CHICK EMBRYO

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Abstract
SUMMARY: Histological examination (azan method) of the islets of Langerhans of the chick embryo showed that β cells were present on the 11–12th day of incubation, but it was difficult to be certain that granules were present in the cells on those days. An occasional granulated β cell could be seen on the 12th day but granulation became general by the 16th day. Histochemical tests (by the Barrnett & Seligman technique) indicated the presence of protein-bound sulphydryl groups in the β cells from the 14th day of incubation onwards. Bioassays of the pancreas by the rat diaphragm method showed that insulin was, in fact, present from the 12th day of incubation onwards. There is no evidence for the presence of insulin in egg yolk, since yolk extracts did not enhance the glucose uptake of rat diaphragm. Alloxan did not inhibit the development of β cells in the pancreas of chick embryos.

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