Experiments on Corneal Preservation (Short-Time Storage)

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Abstract
56 human eyes were stored in a moist chamber from 0 to 4 days at +4°C. 20 human corneal buttons with a scleral rim were stored in the M-K medium for 5 and 7 days at +4°C. After this procedure in both series the temperature reversal effect was measured and the living endothelial cells were counted after being stained with trypan blue and rose bengal. All eyes showed a typical swelling-shrinking phenomena but after 4 days in a moist chamber this effect was definitely less characteristic than in all other periods of storage time. In respect to the endothelial cell counts 5 day M-K medium storage corresponds to 2 day moist chamber storage, and 7 day M-K storage is comparable to 3 day moist chamber storage. The total survival time of the endothelial cells in both series is almost equal.