The extrachromosomal control of nonsense suppression in yeast: An analysis of the elimination of [psi +] in the presence of a nuclear gene PNM -

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Abstract
When a [psi -] strain of yeast mutates to [psi +], the efficiency of suppression by certain ochre suppressors is increased. The [psi +] phenotype is inherited extrachromosomally. There is a nuclear gene, PNM, which, when mutant, causes loss of the [psi +] phenotype. PNM - is dominant to PNM + and a heterozygous diploid gradually loses the ability over successive generations, to produce PNM + [psi +] spores. This paper describes the kinetics of this elimination and the data obtained are discussed in relation to two models of the molecular nature of the [psi] genetic determinant—one considering the [psi] determinant as an autonomous nucleic acid, the other treating the possibility that the [psi] nucleic acid is that which codes for rRNA in the nuclear genome.