Hydrolysis of Crystalloid Storage Protein in Castor Bean Seed Endosperm During and Following Germination

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Abstract
Hydrolysis of the insoluble crystalloid storage proteins of castor bean endosperm during germination released buffer-soluble polypeptides with molecular weights in the presence of sodium dodecyl sulphate of 30000–40000. These polypeptides appear to be dimers since the addition of 2-mercaptoethanol decreases their molecular weights to 15000–22000. Hydrolysis of the crystalloid proteins was detected 12–18 h after seed imbibition (HAI), which is before the completion of germination; maximum rates were attained at 30 HAI. During this period, parallel increases in free amino acids were observed. Hydrolysis of the crystalloid proteins during early germination was insensitive to cycloheximide treatment and therefore did not require newly synthesized proteases. Hydrolysis was effected by proteases which were made in an inactive form during seed development and activated upon seed imbibition.

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