DESENSITIZATION IN VITRO - SPECIFIC INHIBITION BY ANTIGEN OF PASSIVE TRANSFER OF DELAYED HYPERSENSITIVITY BY PERITIONEAL EXUDATE CELLS

  • 1 January 1967
    • journal article
    • research article
    • Vol. 13  (5) , 469-+
Abstract
A preliminary experiment showed that the injection of bovine gamma-globulin into guinea-pigs with delayed hypersensitivity to bovine gamma-globulin reduced the 24-hr. skin reactions to bovine gamma-globulin and (to a lesser extent) PPD [purified protein derivative]. The peritoneal exudate cells from the desensitized donors had a reduced ability to transfer delayed hypersensitivity to bovine gamma-globulin but a normal ability to transfer delayed hypersensitivity to PPD. Likewise, it was possible to diminish the passive transfer of delayed hypersensitivity to bovine gamma-globulin by peritoneal exudate cells, by exposure of the cells to bovine gamma-globulin in vitro. The recipients were tested immediately after cell transfer. This in vitro desensitization was specific, in that the transfer of delayed hypersensitivity to PPD was unaffected. Exposure of cells in vitro to hypotonic conditions and antibody to guinea-pig gamma-globulin did not prevent the passive transfer of delayed hypersensitivity.