A SIMPLE METHOD FOR THE EXTRACTION OF POLYSACCHARIDE-B FROM BRUCELLA CELLS FOR USE IN THE RADIAL IMMUNODIFFUSION TEST DIAGNOSIS OF BOVINE BRUCELLOSIS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 12  (1) , 35-39
Abstract
In order to extract polysaccharide B of Brucella, used in the radial immunodiffusion test (RID) for detection of infected cattle, a quick, simple and safe method was proposed. Phenol killed cells of B. melitensis 16 M, known to produce polysaccharide B, were used. After autoclaving Brucella resuspended in saline, the cells were separated by centrifugation; the polysaccharide B was obtained by 2 successive ethanol precipitations of the supernatant. Comparison of the results of RID tests carried out with the polysaccharide extract of B. melitensis 16 M (rapid method) and 231 sera of infected cattle indicated the validity of this method of preparation. Differences in yield (1.5% for the 2nd precipitate vs. 0.5% for the polysaccharide-reference) and in optimum dose used in the reaction (10 .mu.g vs. 200 .mu.g) revealed that as many as 60 times more RID tests can be made with the polysaccharide obtained by the rapid method.

This publication has 0 references indexed in Scilit: