Circular Dichroism of Hemocyanin
- 1 June 1968
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 63 (6) , 725-729
- https://doi.org/10.1093/oxfordjournals.jbchem.a128837
Abstract
Circular dichroic (CD**) spectra of octopus vulgaris (Japanese form) hemocyanin were measured over a wavelength range of 210 to 700 mμ in the forms of oxy-, deoxy- and apo-hemocyanin. Oxyhemocyanin showed three bands in the visible region (a positive band above 700 mμ, a negative band at 570 mμ, and a positive band at 450 mμ), a large negative band at 350 mμ, several positive maxima in the ultraviolet region and a negative trough at 222 mμ. In the CD spectra of deoxy- and apo-hemocyanin, characteristic three bands in the visible region and a large band at 350 mμ completely disappeared, but the bands in the ultraviolet region still remained. A little change in the CD spectrum was observed in the ultraviolet region on oxygenation of deoxyhemocyanin.Keywords
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