Loci of radiation sensitivity in Bs strains of Escherichia coli

Publisher Website
Google Scholar
Abstract
The genes responsible for u.v. sensitivity in ten sensitive mutants ofE. colistrain B (Bs strains of R. Hill) have been mapped by transduction. Theuvrgenes of all the mutants able to reactivate u.v.-irradiated phage (HCR+), including Bs2, 4, 5, 6, 7, 9 and 10, were linked, 50–95%, withmalB. The gene of Bs12 (HCR) was also linked tomalBas isuvr A. The gene of Bs8 (HCR) was linked togal, that of Bs3 (HCR) tohis. Transduction ofmal+from a strain withuvr A, or amal+derivative of Bs12, to Bs2, 4, 5, 6, 7, 9 or 10, yielded about 30% u.v.-resistant transductants. Amal+transduction with Bs2mal+as donor and other Bs strains as recipients yielded < 0·1% u.v.-resistant transductants. ThemalB-linkeduvrgenes of all mutants (except Bs3 and Bs8) were transducible withmetA. The quasi-reciprocal crosses and three-point tests suggested the order of markers asmetA malB uvr(HCR+)uvr(HCR). The sensitivity gene of Bs9 was exceptional in that it appeared to lie betweenmetAandmalB. The sensitivity gene of Bs11 could not be mapped because transductants to that strain were not possible, nor did it act as a recipient in sexual recombinations.

This publication has 24 references indexed in Scilit: