IMMUNOGENICITY AND PROTECTIVE ACTIVITY OF GLYCOPROTEIN EXTRACTED FROM RABIES VIRUS - RESULTS FROM COMPARISON OF PREPARATIONS OBTAINED BY DIFFERENT METHODS OF PURIFICATION

  • 1 January 1976
    • journal article
    • research article
    • Vol. B127  (2) , 257-267
Abstract
A rabies vaccine was prepared from virus grown on primary cultured bovine fetal kidney cells. After .beta.-propiolactone inactivation of the virus, the vaccine was partially purified by zinc acetate precipitation, G-75 Sephadex chromatography and ultracentrifugation prior to a non ionic detergent solubilization. A glycoprotein fraction with protective capacity was extracted by Triton X100 from the spikes of the inactivated rabies virus. An electrophoretically homogenous glycoprotein had higher values of protective activity compared to the initial tissue culture vaccine. The yield from this extraction method was 10-20%, based upon an immunological assay.

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