Amino Acid Requirements of Young Women Based on Nitrogen Balance Data II. Studies on Isoleucine and on Minimum Amounts of the Eight Essential Amino Acids Fed Simultaneously

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Abstract
The amount of isoleucine required to maintain nitrogen equilibrium in 7 healthy young women has been determined. When these subjects were placed on a diet containing 6 to 7 gm of nitrogen in the form of the essential L-amino acids and glycine, it was found that the isoleucine requirement varied from 250 to 450 mg per day. Three of the subjects required 250 mg, three 350 mg, and one 450 mg. In the second part of the experiment, minimum required amounts of all 8 essential amino acids were fed simultaneously to 6 subjects. The remainder of the nitrogen content of the diet was chiefly glycine. It was found that nitrogen balance was maintained in these subjects for the experimental period of 5 days with the exception that equivocal results were obtained in one individual.