The glucose distribution in 9l rat brain multicell tumor spheroids and its effect on cell necrosis
- 15 November 1982
- Vol. 50 (10) , 2066-2073
- https://doi.org/10.1002/1097-0142(19821115)50:10<2066::aid-cncr2820501017>3.0.co;2-x
Abstract
The glucose consumption rate of 9L rat brain tumor cells was determined as a function of concentration. Glucose uptake followed Michaelis‐Menten kinetics with a Km of 0.58 mM and a vmax of 1.6 pg/cellmin. The glucose diffusion coefficient in spheroids of 9L tumor cells was determined to be 1.5 × 10−6 cm2/s at 37°C. Using these parametric values, the glucose distribution in 9L multicell spheroids was calculated and related to the viable and necrotic zones.Keywords
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