The Relative Quantities of the Heat-Stable and Heat-Labile Fractions of Vitamin B in Raw and Evaporated Milk

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Abstract
Biological analyses by three different methods have shown that commercial evaporation of cows' milk destroys about one-sixth to one-fifth of the antineuritic heat-labile fraction. All cows' milk seems to be quite low in this factor however, 25 cc. 3.49 gm. solids being required per rat per day for optimum growth. Milks do not apparently vary widely in their antineuritic content as purchased on the market. There is no identifiable destruction of the heat-stable, growth-promoting fraction during commercial evaporation. Milk is capable of adequately supplying this fraction for optimum growth. The amount necessary, while lower than that required for the antineuritic factor, is also fairly large, that is 17 to 20 cc. 2.37 to 2.78 gm. solids. The question of the identity of this heat-stable factor with the antipellagra fraction is still unsettled. The authors gratefully acknowledge the assistance of Miss Kathryn Knowlton in carrying out some of the quantitative feeding experiments. They also desire to express their appreciation for the coöperation given by the Evaporated Milk Association and the Carnation Milk Products Co.

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