An investigation of the mechanism of activation of tryptophan by tryptophanyl‐tRNA synthetase from beef pancreas
- 1 February 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 138 (3) , 597-602
- https://doi.org/10.1111/j.1432-1033.1984.tb07956.x
Abstract
Adenosine (S)-5''-[.alpha.-17O, 18O2]triphosphate was synthesized and used to investigate the stereochemical course of activation of tryptophan by tryptophanyl-tRNA synthetase from beef pancreas. The reaction proceeds by displacement of Mg pyrophosphate from MgATP with inversion of configuration at P.alpha.. Tryptophanyl-tRNA synthetase catalyzes positional isotope exchange in adenosine 5''-[.beta.-18O2]triphosphate in the presence of tryptophan but not in its absence or in the presence of the competitive inhibitors tryptamine and tryptophanol. These observations eliminate a multi-adenylyl transfer or dissociative mechanism and in combination with the stereochemical study leave a direct associative in line displacement of P.alpha. of MgATP as the only tenable mechanism for the activation of tryptophan by tryptophanyl-tRNA synthetase.This publication has 33 references indexed in Scilit:
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