Induction of selective phosphorylation of a fat body protein of Sarcophaga peregrina larvae by 20-hydroxyecdysone
- 15 April 1985
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 227 (2) , 683-688
- https://doi.org/10.1042/bj2270683
Abstract
20-Hydroxyecdysone was shown to induce selective phosphorylation of a fat body protein of Sarcophaga peregrina larvae with a molecular mass of 30 kDa. This phosphorylation was not associated with synthesis of new protein. Fractionation of 32P-labelled fat body by differential centrifugation showed that this protein was mainly present in the membrane-rich fraction, although we could not specify the membrane. Thus, 20-hydroxyecdysone may modify the function of the fat body by inducing phosphorylation of a specific membrane protein.This publication has 21 references indexed in Scilit:
- Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.Proceedings of the National Academy of Sciences, 1978
- Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytesDevelopmental Biology, 1977
- Uptake of β-ecdysone by the fat body and membrane vesicles of fat body cells of Sarcophaga peregrina larvaeJournal of Insect Physiology, 1976
- Selective activation of RNA polymerase I in fat body nuclei of Sarcophaga peregrina larvae by β-ecdysoneDevelopmental Biology, 1976
- The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated.1974
- The stimulation of the phosphorylation of ribosomal protein S6 by cycloheximide and puromycinBiochemical and Biophysical Research Communications, 1974
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The site of diphosphoinositide synthesis in rat liverBiochemical and Biophysical Research Communications, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951