Abstract
Bovine red blood cells (RBC) were made to entrap homidium bromide, a trypanocidal drug. Hypotonic dialysis resulted in 0.3 mg drug encapsulated per milliliter of RBC. Homidium bromide had no untoward effects on the encapsulation of a marker14C-sucrose. However, RBC containing homidium bromide had a mean cell volume (MCV) larger than that of sham-loaded cells. EDTA did not reverse the effects of the drug on the MCV. The in vitro drug efflux rate was 0.097 mg/h. The effects of drug on the 24-h circulating survival was determined, and 24-h cell survival was reduced from 80% to 50%.

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