PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus
- 7 April 2000
- journal article
- rhythms
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 11 (5) , 907-912
- https://doi.org/10.1097/00001756-200004070-00002
Abstract
Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-β1 and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the M1-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-β1 but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN.Keywords
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