Effect of an anti-oestrogen on implantation of mouse blastocysts

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Abstract
Mouse blastocysts collected on day 4 were cultured for 4 h in Whitten''s medium containing 5 .mu.g CI 625 [1-[2-[p-[.alpha.-(p-methoxyphenyl)-.beta.-nitrostyryl]phenoxy]ethyl]pyrrolidine monocitrate], a specific estrogen antagonist, and transferred to the uteri of pseudopregnant hosts. There was a significant reduction in the number of implantations observed on day 11 when compared with those from blastocysts which were similarly transferred after culture in medium alone or in medium containing 1 .mu.g estradiol-17.beta. and 5 .mu.g CI 628. This is further evidence for the involvement of embryonic estrogen in implantation.