Proposed Standards for Alkaline Phosphatase in Pasteurized Milk Determined by an Automated Procedure

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Abstract
A standard of 4 μg para-nitrophenol per ml is proposed for alkaline phosphatase in pasteurized milk tested by the AutoAnalyzer method based on comparison with the modified spectrophotometric and Kay and Graham methods. Using Versatol E as a standardized alkaline phosphatase source, an equivalent standard of 2.8 BLB* units (1.88 x Bessey-Lawry-Brack International Units) is proposed. When activities are expressed in milli-Units per ml (milli-μMoles of para-nitrophenol per ml per min), a standard of 2.5 mU per ml is proposed for pasteurized milk. The precision between seven laboratories was greater when activities were expressed in μg para-nitrophenol per ml or mU per ml than in BLB* units, and when the concentration of a pooled raw milk sample (100 herds) exceeded 0.25%. All laboratories reported a 0.25% raw milk sample as pasteurized and a 0.5% raw milk sample as under-pasteurized by the three proposed standards.

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