SPLENIC IMAGING WITH TC-99M-LABELED ERYTHROCYTES - COMPARATIVE-STUDY OF CELL-DAMAGING METHODS

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 17  (12) , 1038-1043
Abstract
The RBC [red blood cells] from donor animals were labeled with 99mTcO4- and damaged by heat, excess acid citrate dextrose (ACD), excess Sn(II) ion, or the sulfhydryl inhibitors N-ethylmaleimide (NEM) or p-hydroxymercuribenzoate (PMB). The organ distributions of undamaged and damaged RBC were determined in rats, and splenic imaging studies were performed in rabbits. Splenic deposition and spleen-to-liver ratios with heat- or sulfhydryl-damaged 99mTc-RBC were significantly greater (P < 0.001) than the values obtained using ACD or Sn(II) ion. Heat-damaging produces good splenic localization of 99mTc-RBC but requires rigidly controlled incubation conditions. NEM-damaging provides an excellent and predictable alternative approach.