Determination of Factors of the Kinin System in Rat Plasma

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Abstract
Methods were developed for determining the kininogen fractions, kininase and prekallikrein. The plasma prekallikrein was activated by 20 % (v/v) acetone for about 17 hours (20–24°). Urine kallikrein was prepared by dialysis of urine against running tap water for about 24 hours. Kininase activity was eliminated in plasma, plasma kallikrein and urine kallikrein by incubation at 37° with EDTA‐2Na (1.0 × 102 M) for about 24 hours. Kinin assays were carried out on the isolated rat uterus. Released kinin was calculated as μg bradykinin/ml plasma. The total kininogen, whether determined by activation with acetone (16 % v/v for not less than 5 hours) and subsequent incubation with plasma kallikrein, by incubation with plasma kallikrein and then urine kallikrein, or by incubation with acetone (20 % v/v for 17 hours) and subsequent evaporation of the acetone, was found to be the same, 2.0 μg/ml plasma as an average value of 7 plasma batches corresponding to a total of 90 rats (S. D. = 0.09). The average values of kinin released by incubation with plasma kallikrein and by urine kallikrein were 1.5 μg/ml and 1.4 μg/ml plasma respectively with S. D. values of 0.11 and 0.06 respectively. The procedures for kininase and for prekallikrein determinations corresponded closely to previously published methods for estimation of the same parameters in human plasma (RINVIK, DYRUD & BRISEID 1966; BRISEID, DYRUD & ARNTZEN 1968).

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