Adenosine-induced bacteriostasis in Micrococcus sodonensis

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Abstract
In a synthetic growth medium at a concentration of 1.0 mM, adenosine had a marked bacteriostatic effect on the growth of Micrococcus sodonensis. With the exceptions of hypoxanthine and inosine, which were slightly inhibitory, other purine bases and ribonucleosides did not inhibit the growth of the organism. When incubation of adenosine-supplemented cultures was prolonged, the organism was able to recover from the growth inhibition after a period of time which varied directly with the initial adenosine concentrations. Chromatographic analysis of the supernatants of those cultures revealed that exogenous adenosine concentrations declined steadily and the termination of the inhibited phase of growth correlated with the exhaustion of exogenous adenosine.It was observed that de novo biosynthesis of thiamine was inhibited in adenosine-supplemented cultures and that this inhibition was also relieved upon the exhaustion of exogenous adenosine. Bacteriostasis could be prevented by including either thiamine or its pyrimidine precursor (B1-pyrimidine) in adenosine-supplemented cultures and it was concluded that the observed growth inhibition reflected a depletion of the intracellular thiamine pool resulting from an adenosine-associated inhibition of B1-pyrimidine biosynthesis.

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