RAS Function and Protein Kinase Cascades
- 28 September 2007
- book chapter
- Published by Wiley
- Vol. 176, 53-66
- https://doi.org/10.1002/9780470514450.ch4
Abstract
This paper reviews recent progress in understanding the function of RAS in three systems: the budding yeast (Saccharomyces cerevisiae), the fission yeast (Schizosaccharomyces pombe) and Xenopus laevis oocytes. One of the functions of RAS in S. cerevisiae is the stimulation of adenylate cyclase. This leads to the activation of the cAMP-dependent protein kinases--a function that has probably not been conserved in evolution. The immediate function of RAS in S. pombe is not known, but it may lead to the activation of a protein kinase cascade. This cascade has likely been conserved in evolution and linkage between it and RAS can be demonstrated in cell-free extracts from Xenopus oocytes. The Xenopus cell-free system provides a means to test specific hypotheses about RAS function and to isolate targets of RAS.Keywords
This publication has 67 references indexed in Scilit:
- Activation of the MAP kinase pathway by the protein kinase rafCell, 1992
- Adenylyl cyclasesCell, 1992
- Involvement of p21ras in activation of extracellular signal-regulated kinase 2Nature, 1992
- ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSKCell, 1992
- ASP‐56, a new actin sequestering protein from pig platelets with homology to CAP, an adenylate cyclase‐associated protein from yeastFEBS Letters, 1992
- Gap Domains Responsible for Ras P21-Dependent Inhibition of Muscarinic Atrial K + Channel CurrentsScience, 1992
- Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.Genes & Development, 1991
- STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.Genes & Development, 1990
- S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating proteinCell, 1990
- Requirement of either of a pair of ras-related genes of Saccharomyces cerevisiae for spore viabilityNature, 1984