USE OF „REPORTER GROUPS” IN STRUCTURE-FUNCTION STUDIES OF PROTEINS
- 1 October 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (4) , 1017-1024
- https://doi.org/10.1073/pnas.52.4.1017
Abstract
A new technique has been developed for the correlation of function with structure in protein molecules. A group which is sensitive to changes in environment and which can transmit a signal to an appropriate detector is introduced into a specific position in the protein. This group may report changes in its environment caused either by direct interaction with substrate or by a conformational alteration in the protein structure. An application of this method is described in which the compound 2-bromoacetamido-4-nitrophenol is reacted with the methionine residue near the active serine of chymotrypsin. On mixing substrates and inhibitors with the modified protein, character -istic difference spectra are obtained. Some of the applications and limitations of the method are discussed.Keywords
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