Residues 1 to 80 of the N‐terminal domain of the β subunit confer neuronal bungarotoxin sensitivity and agonist selectivity on neuronal nicotinic receptors
- 11 October 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 332 (1-2) , 139-142
- https://doi.org/10.1016/0014-5793(93)80500-t
Abstract
Standard two electrode voltage clamp techniques were used to investigate the response of neuronal nicotinic acetylcholine receptors, expressed in Xenopus oocytes, to various agonists and neuronal bungarotoxin (NBT). The β subunit is an important determinant of the receptor's pharmacological profile. Co-expression of α4 and β2 subunits produced a receptor that was relatively insensitive to cytisine and nicotine and inhibited by NBT, whilst the α4β4 combination produced a receptor that was highly sensitive to cytisine and nicotine but resistant to toxin. The first 80 amino acids of the N-terminal domain of the β subunit are implicated in these characteristics, since the combination of α4 with a hybrid β subunit comprising amino acids 1 → 80 of β2 and 81 → 416 of β4 became relatively insensitive to nicotine and cytisine and resistant to inhibition by neuronal bungarotoxin.Keywords
This publication has 24 references indexed in Scilit:
- The amino terminal half of the nicotinic β-subunit extracellular domain regulates the kinetics of inhibition by neuronal bungarotoxinProceedings Of The Royal Society B-Biological Sciences, 1993
- The Diversity of Neuronal Nicotinic Acetylcholine ReceptorsAnnual Review of Neuroscience, 1993
- Pharmacological and functional diversity of neuronal nicotinic acetylcholine receptorsTrends in Pharmacological Sciences, 1991
- Pentameric structure and subunit stoichiometry of a neuronal nicotinic acetylcholine receptorNature, 1991
- Neurotoxins Distinguish Between Different Neuronal Nicotinic Acetylcholine Receptor Subunit CombinationsJournal of Neurochemistry, 1990
- Functional properties and developmental regulation of nicotinic acetylcholine receptors on embryonic chicken sympathetic neuronsNeuron, 1989
- Single-channel currents of rat neuronal nicotinic acetylcholine receptors expressed in xenopus oocytesNeuron, 1989
- The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: β4Neuron, 1989
- Distribution of alpha2, alpha3, alpha4, and beta2 neuronal nicotinic receptor subunit mRNAs in the central nervous system: A hybridization histochemical study in the ratJournal of Comparative Neurology, 1989
- Kappa-Bungarotoxin: a probe for the neuronal nicotinic receptor in the avian ciliary ganglionBrain Research, 1983