The mode of action of alpha sarcin and a novel assay of the puromycin reaction
- 15 January 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 170 (1) , 57-61
- https://doi.org/10.1042/bj1700057
Abstract
A new technique was developed for measuring the amount of peptidyl-tRNA in a protein-synthesizing system in vitro. By this technique the course of the puromycin reaction may be followed and the modes of action of various inhibitors of protein synthesis readily determined. We conclude that the polypeptide alpha sarcin inhibits the binding of aminoacyl-tRNA into the ribosomal ‘A’ site, that sparsomycin inhibits the peptidyl transferase reaction and that cycloheximide may block translocation.This publication has 13 references indexed in Scilit:
- Comparative studies on two reaction center preparations from Rhodopseudomonas speheroides YFEBS Letters, 1974
- Initiation of protein synthesis: Evidence for messenger RNA-independent binding of methionyl-transfer RNA to the 40 S ribosomal subunitJournal of Molecular Biology, 1973
- Control of globin synthesis: The role of hemeJournal of Molecular Biology, 1972
- The mode of action of thiostrepton in vivoBiochemical and Biophysical Research Communications, 1971
- Inhibition of Peptide Initiation on Reticulocyte Ribosomes by EdeineEuropean Journal of Biochemistry, 1971
- The mechanism of cycloheximide inhibition of protein synthesis in rabbit reticulocytesBiochemical and Biophysical Research Communications, 1969
- Bacterial protein synthesis: The effects of antibioticsJournal of Molecular Biology, 1967
- Inhibition by Sparsomycin and Other Antibiotics of the Puromycin-Induced Release of Polypeptide from Ribosomes*Biochemistry, 1967
- Inhibition of protein synthesis in reticulocytes by antibiotics: III. Mechanism of action of sparsomycinBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- The puromycin reaction and its relation to protein synthesisJournal of Molecular Biology, 1964