Expression of TAR RNA-binding protein in baculovirus and co-immunoprecipitation with insect cell protein kinase
- 1 October 1995
- journal article
- Published by Springer Nature in Journal of Biomedical Science
- Vol. 2 (4) , 322-329
- https://doi.org/10.1007/bf02255219
Abstract
TAR RNA-binding protein TRBP was originally isolated by its binding affinity for radiolabeled HIV-1 leader RNA. Subsequent studies have suggested that this protein is one member of a family of double-stranded RNA-binding proteins. Recent findings indicate that TRBP might function to antagonize the translational inhibitory effect that can be mediated through cellular protein kinase, PKR. Here, we report on the over-expression of a cDNA coding for TRBP in eukaryotic SF9 cells using baculovirus. We characterized the nuclear localization of TRBP in insect cells, and we demonstrate that TRBP co-immunoprecipitates with a protein in these cells antigenically related to human PKR.Keywords
This publication has 44 references indexed in Scilit:
- Posttranscriptional regulation and RNA binding proteins in developmentJournal of Biomedical Science, 1995
- CONTROL OF RNA INITIATION AND ELONGATION AT THE HIV-1 PROMOTERAnnual Review of Biochemistry, 1994
- Direct interaction of human TFIID with the HIV-1 transactivator TatNature, 1994
- Lupus Autoantigen Ku Protein Binds HIV-1 TAR RNA in VitroBiochemical and Biophysical Research Communications, 1993
- PKR: Proposed Nomenclature for the RNA-Dependent Protein Kinase Induced by InterferonJournal of Interferon Research, 1993
- Two RNA-binding motifs in the double-stranded RNA-activated protein kinase, DAI.Genes & Development, 1992
- Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin.Genes & Development, 1991
- Characterization of a Human TAR RNA-Binding Protein That Activates the HIV-1 LTRScience, 1991
- Regulation of HIV expressionAIDS, 1991
- Activation of double-stranded RNA-dependent kinase (dsl) by the TAR region of HIV-1 mRNA: A novel translational control mechanismCell, 1989