Quantitative Mass Spectrometry Identifies Insulin Signaling Targets in C. elegans
Top Cited Papers
- 3 August 2007
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 317 (5838) , 660-663
- https://doi.org/10.1126/science.1139952
Abstract
DAF-2, an insulin receptor–like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.Keywords
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