Photochemical Generation of Ribose Abasic Sites in RNA Oligonucleotides
- 23 March 2005
- journal article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 7 (8) , 1493-1496
- https://doi.org/10.1021/ol050120h
Abstract
A general method for the photochemical generation of ribose abasic sites within RNA oligonucleotides is reported. Photochemically caged nucleoside phosphoramidite analogues were prepared and incorporated into RNA oligonucleotides by automated RNA synthesis. Irradiation of the modified RNA at 350 nm efficiently produced ribose abasic sites at specific sites within RNA sequences. The current approach offers a chemical route to RNA abasic lesions for RNA biochemical studies.Keywords
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