Ion-Channel Gating in Transmembrane Receptor Proteins: Functional Activity in Tethered Lipid Membranes
- 1 February 1999
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition in English
- Vol. 38 (3) , 389-392
- https://doi.org/10.1002/(sici)1521-3773(19990201)38:3<389::aid-anie389>3.0.co;2-u
Abstract
Through thiolipids a planar lipid bilayer (1) was immobilized on a gold support (2) for use as an electrode. This allows the detection of the ligand-gating function of the natural transmembrane channel protein OmpF (3) reconstituted in the artificial membrane: the binding of a domain (4) of the toxin colicin N, observed by surface plasmon resonance, induces the blocking of the OmpF channel protein, as shown by impedance spectroscopy.This publication has 0 references indexed in Scilit: