ISOLATION AND CHARACTERIZATION OF MICROBODIES FROM CANDIDA TROPICALIS PK 233 CELLS GROWN ON NORMAL ALKANES

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Abstract
Numerous microbodies developed at the logarithmic phase of growth in cells of C. tropicalis when cultures were grown in a medium containing normal alkanes. From the homogenate of protoplasts prepared from 16-h cells of Candida, biologically active microbodies were isolated by discontinuous sucrose density gradient centrifugation. The fractions recovered in the middle and lower-density regions of the gradient were associated with substantially high activities of 3 peroxisomal marker enzymes, catalase, D-amino acid oxidase and urate oxidase, while a mitochondrial marker enzyme, cytochrome oxidase, was only slightly detected. EM studies revealed that these fractions were largely comprised of morphologically well-preserved microbodies with a smaller number of more or less degraded organelles, but were virtually devoid of mitochondria. The microbody fractions contained an appreciable amount of DNA.