Triple Helix-Specific Ligands
- 19 June 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 256 (5064) , 1681-1684
- https://doi.org/10.1126/science.256.5064.1681
Abstract
A triple helix is formed upon binding of an oligodeoxynucleotide to the major groove of duplex DNA. A benzo[e]pyridoindole derivative (BePI) strongly stabilized this structure and showed preferential binding to a triplex rather than to a duplex. Energy transfer experiments suggest that BePI intercalates within the triple helix. Sequence-specific inhibition of transcription initiation of a specific gene by Escherichia coli RNA polymerase by a triplexforming oligodeoxynucleotide is strongly enhanced when the triplex is stabilized by BePI. Upon irradiation with ultraviolet light, BePI induces covalent modifications of the target within the triple helix structure.Keywords
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