A Preliminary Report on Histochemography

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Abstract
A series of Eastman NTB photographic plates were exposed directly to peripheral blood, spleen, kidney, liver, heart, and lung of a normal rat sacrificed in dark room under red light; the sections of the tissues were placed in direct contact with the emulsion and after 10-40 min. were stored in this condition at -15[degree]C for various periods of time. They were then developed using diluted Eastman Kodak D19. In all cases the developed plates showed patterns where they were exposed to the tissues, and some of the patterns were in good correspondence with the architecture of the tissue. Tissues frozen before being placed on plates gave the same results. If a glass coverslip or 1 mm. thick quartz was placed between the tissue and the emulsion, no patterns were developed indicating the effect to be chemical, and not due to emission of photons. Tissues fixed in Bouin''s did not give[image] the effect. A warning is given concerning the interpretations of radioautographs made under conditions in which fresh tissue is placed in direct contact with photographic emulsion.

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