The structural protein ODV-EC27 of Autographa californica nucleopolyhedrovirus is a multifunctional viral cyclin
- 15 September 1998
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 95 (19) , 11205-11210
- https://doi.org/10.1073/pnas.95.19.11205
Abstract
Two major characteristics of baculovirus infection are arrest of the host cell at G2/M phase of the cell cycle with continuing viral DNA replication. We show that Autographa californica nucleopolyhedrovirus (AcMNPV) encodes for a multifunctional cyclin that may partially explain the molecular basis of these important characteristics of AcMNPV (baculovirus) infection. Amino acids 80–110 of the viral structural protein ODV-EC27 (−EC27) demonstrate 25–30% similarity with cellular cyclins within the cyclin box. Immunoprecipitation results using antibodies to −EC27 show that −EC27 can associate with either cdc2 or cdk6 resulting in active kinase complexes that can phosphorylate histone H1 and retinoblastoma protein in vitro. The cdk6-EC27 complex also associates with proliferating cell nuclear antigen (PCNA) and we demonstrate that PCNA is a structural protein of both the budded virus and the occlusion-derived virus. These results suggest that −EC27 can function as a multifunctional cyclin: when associated with cdc2, it exhibits cyclin B-like activity; when associated with cdk6, the complex possesses cyclin D-like activity and binds PCNA. The possible roles of such a multifunctional cyclin during the life cycle of baculovirus are discussed, along with potential implications relative to the expression of functionally authentic recombinant proteins by using baculovirus-infected cells.Keywords
This publication has 60 references indexed in Scilit:
- Cyclin encoded by KS herpesvirusNature, 1996
- BPV E1 protein alters the kinetics of cell cycle entry of serum starved mouse fibroblastsCytometry, 1995
- The pk-1 Gene of Autographa Californica Multinucleocapsid Nuclear Polyhedrosis Virus Encodes a Protein KinaseJournal of General Virology, 1994
- p21 is a universal inhibitor of cyclin kinasesNature, 1993
- Universal control mechanism regulating onset of M-phaseNature, 1990
- Viral proliferating cell nuclear antigenNature, 1989
- Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis.The Journal of cell biology, 1988
- Intranuclear membranes and the formation of the first meiotic spindle in Xenos peckii (Acroschismus wheeleri) oocytes.The Journal of cell biology, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970