A Whole Blood Solubility and Centrifugation Test for Sickle Cell Hemoglobin: A Clinical Trial

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Abstract
A rapid whole blood solubility and centrifugation test for the detection of sickle cell hemoglobin was recently described. The method is based on the insolubility of reduced sickle cell hemoglobin, but is capable of distinguishing the sickle cell trait from sickle cell anemia and hence represents an advance over existing solubility tests. The performance of this test has been assessed in 400 blood samples, which included a variety of sickle hemoglobinopathy conditions. Samples were simultaneously checked by starch gel electrophoresis. The solubility test correctly indicated the presence of hemoglobin S in all cases and, depending on the amount of soluble hemoglobin, permitted a distinction between cases containing approximately 50% hemoglobin S(AS and SC) and those containing higher concentrations (SS and sickle thalassemia). The test proved to be inexpensive, reliable, and rapid, and is well suited to screening programs for large populations.

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