Calcium mobilization and Na+/H+ antiport activation by endothelin in human skin fibroblasts
- 9 October 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 256 (1-2) , 38-42
- https://doi.org/10.1016/0014-5793(89)81713-2
Abstract
Endothelin (ET-1) has been shown to exert vasoconstrictor activity in vivo and mobilize Ca2+ in vascular smooth muscle cells in culture. In this paper we show that the human skin fibroblast exhibits specific receptors to ET-1 and that activation of these receptors results in increased intracellular Ca2+ (Ca2+i) and accelerated Na+/H+ antiport activity. ET-1 raised Ca2+i in a dose-response manner; the peak Ca2+i rise was from basal levels of 112.2 ± 21.9 to 299.2 ± 49.7 nM at 300 nM ET-1. This rise was attenuated by removal of extracellular Ca2+i0. Although ET-1 did not alter basal intracellular pH, it enhanced Na+/H+ antiport activity of acidified cells. Fibroblasts demonstrated 156 ± 18 (mean ± SE) ET-1 receptors per unit cell and an equilibrium dissociation constant of 203.4 ± 35.6 pM. Inasmuch as ET-1 plays a role in the metabolism of cells such as the undifferentiated fibroblast, an important action of this peptide may be to act as a growth factor.Endothelin; Growth factor; (Human skin fibroblastKeywords
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